Sorbitol-gelatin and glutamic acid-lactose solutions for stabilization of reference preparations of measles virus.

نویسندگان

  • E de Rizzo
  • E C Tenório
  • I F Mendes
  • F L Fang
  • M M Pral
  • C S Takata
  • C Miyaki
  • N M Gallina
  • H N Tuchiya
  • O K Akimura
چکیده

The work reported here sought to assess the protection afforded by two stabilizing solutions (sorbitol-gelatin and glutamic acid-lactose) in preserving the potency of freeze-dried Schwarz strain measles virus during storage with a view to the production of reference preparations and working lots of virus suspensions. Stabilized virus suspensions and control suspensions were stored at -70 degrees C or were freeze-dried and stored at -20 degrees C, and their potency was determined over a storage period of 21 months. It was found that the sorbitol-gelatin imparted more satisfactory stability (r = +0.18) to the freeze-dried virus suspensions than did the glutamic acid-lactose. The results also indicate that sorbitol-gelatin, used under the conditions of this study, is an effective stabilizer in the preparation of freeze-dried suspensions of Schwarz strain measles virus employed as reference preparation working lots.

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عنوان ژورنال:
  • Bulletin of the Pan American Health Organization

دوره 23 3  شماره 

صفحات  -

تاریخ انتشار 1989